Structural and Functional Studies on Photoactive Retinal Proteins: Light Becomes Drugs with Proteins

Time-resolved structural studies with serial crystallography: A new light on retinal proteins

Structural information of the different conformational states of the two prototypical light-sensitive membrane proteins, bacteriorhodopsin and rhodopsin, has been obtained in the past by X-ray cryo-crystallography and cryo-electron microscopy. However, these methods do not allow for the structure determination of most intermediate conformations. Recently, the potential of X-Ray Free Electron La...

Functional and Structural Studies on Heptahelical Membrane Proteins

12 Lyhennelmä (Synopsis in Finnish) 13

Structural Studies on Cytoskeletal Proteins.

A number of componenets of cytoskeleton are very large proteins which are in their complete native form non amenable to the structure determination via X-ray diffraction techniques due to their non-globular shape and intrinsic flexibility which hinder the formation of suitable crystals. Dissecting such proteins into their representative building blocks-domains and subsequent structural studies ...

Structural studies on lysosomal proteins

Structural and functional studies of Porphyromonas gingivalis fimbrial proteins

Porphyromonas gingivalis expresses two forms of fimbriae, FimA and Mfa1. Each fimbria consists of five proteins; FimA-E and Mfa1-5. While the assembly of the type-1 fimbriae from Escherichia coli is well studied; the chaperone-usher pathway, very little is known about the polymerization of P. gingivalis fimbriae. The P. gingivalis fimbrial proteins form lipidated precursors that have an N-termi...